What applications is the Watchmaker mRNA Library Prep Kit suitable for?
- 基因表达分析
- 基因融合检测
- 异构体/选择性剪接分析
- 新型转录本发现
- 单核苷酸变异 (SNV) 检测
- Pathway analysis
Which species are the Watchmaker mRNA Library Prep Kits compatible with?
The Watchmaker mRNA Library Prep Kit is compatible with eukaryotic species.
Why use mRNA enrichment?
mRNA enrichment focuses sequencing on coding transcripts. If your application only requires coverage of coding transcripts, then mRNA enrichment provides improved sequencing economy by focusing on transcripts you care about.
Does the Watchmaker mRNA Library Prep Kit provide strand orientation information?
是，工作流程提供了链 RNA-序列文库。
Does this product support long-read sequencing?
We use random primers to prime during first strand synthesis, which does not support full-length cDNA generation. This makes it unlikely to be compatible with long-read applications.
What sample types are compatible with the Watchmaker mRNA Library Prep Kit?
Intact (RIN >7) RNA ranging from 2.5 – 1000 ng is compatible with the Watchmaker mRNA Library Prep Kit. This includes RNA derived from tissues and cell lines, suspended in 50 µl of RNase-free water.
This kit is not compatible with degraded samples, such as FFPE. To prepare libraries from degraded inputs, please use the Watchmaker RNA Library Prep Kit with Polaris Depletion.
For inputs outside this range, please contact support@watchmakergenomics.com.
RNA 纯度要求是什么？
起始 RNA 应没有污染 DNA。如果总 RNA 含有 DNA，则通过与 DNase I（试剂盒中未提供）一起孵育来去除污染。RNA should be suspended in RNase-free water and be free of salts (e.g., Mg2+, or guanidinium salts), chelating agents (e.g., EDTA or EGTA), and organics (e.g., phenol or ethanol).
Can this kit be used to detect non-coding RNAs?
This kit enriches for coding RNA. To detect non-coding RNAs, please use the Watchmaker RNA Library Prep Kit with Polaris Depletion.
Which adapters are the Watchmaker mRNA Library Prep Kit compatible with?
该试剂盒兼容接头，有一个 3' 悬空 T，以方便 dscDNA 连接期间的接头方向。请注意，接头质量会影响文库的整体制备效率。在使用前，确保接头具有足够的双向性，并具有适当的浓度。要获得市售的接头和更详细的接头建议的列表，请联系 support@watchmakergenomics.com。
Is this kit compatible with other (non-Illumina) sequencing platforms?
This kit has been demonstrated to be compatible with Element, Singular and Ultima sequencing platforms.
涡旋混合对于含有 RNA 和/或酶的反应安全吗？
建议使用涡旋混合来生成预混液并后续添加到样品中。只要注意确保反应完全均匀，移液管混合是一种可接受的替代方法。
保质期和建议的储存条件是什么？
The Watchmaker mRNA Library Prep Kit has a shelf life of 6 months upon receipt. The mRNA capture Kit should be stored at 2 – 8°C and the RNA Library Prep Kit at -25°C to -15°C. Both kits should be handled under recommended conditions.
Which modules are included in the Watchmaker mRNA Library Prep Kit?
The mRNA Library Prep Kit (7BK0001) consists of the mRNA capture kit (7K0105) and RNA Library Prep Kit (7K0078). You will receive two boxes, labeled with part numbers 7K0105 and 7K0078.
What is the purpose of the FFPE Treatment Buffer included in the RNA Library Prep module?
The FFPE treatment buffer in the RNA Library Prep Kit box is not used as part of the mRNA Library Prep Kit workflow. This tube may be removed from the kit and discarded.
What other RNA Library Prep Kits does Watchmaker offer?
The Watchmaker RNA Library Prep Kit with Polaris Depletion removes uninformative ribosomal and globin RNAs allowing for whole transcriptome analysis, including long noncoding RNAs. This kit is compatible with both high quality and degraded RNA samples.
The Watchmaker RNA Library Prep Kit is suitable for total RNA and typically used upstream of target enrichment. This kit is compatible with both high quality and degraded RNA samples.
Can this kit be automated?
The Watchmaker mRNA Library Prep Kit is highly automatable. The kit was developed with automation in mind and 96 reaction kits include generous overages to account for dead volumes on liquid handling platforms. Visit our automation page for a list of scripts already developed or to contact a member of our automation team regarding your platform and needs.
Do you offer custom formats?
Yes! Watchmaker offers customer fills, packaging, concentrations, and labeling – including private label – designed to meet your unique needs. We offer flexible terms to serve organizations of any size, and our right-sized processes enable rapid turnaround time on customization. Please contact sales@watchmakergenomics.com to learn more about our capabilities.
Are you ISO 13485-certified?
Yes! Our Quality Management System has achieved ISO 13485:2016 Certification. 我们的证书被授予设计、开发、制造、合同制造和支持在医学研究中的基因组学应用的高性能试剂。Download the certificate here.
