What is RNase Inhibitor?
RNase Inhibitor exhibits high-affinity, non-competitive binding of RNases A, B, and C, enabling high-quality cDNA synthesis from low-quality RNA samples.
How is one unit of RNase Inhibitor defined?
1 unit of RNase Inhibitor is defined as the amount of RNase Inhibitor required to inhibit activity of 0.375 ng of RNase A by ≥ 95%.
What are the applications for the RNase inhibitor?
- First strand synthesis
- RT-PCR, RT-qPCR
- Nuclei Isolation
- Single-cell RNA-sequencing
- in vitro synthesis (IVT)
- cDNA synthesis
- Cell-free cloning
- RNA labeling
- Any applications where maintaining RNA integrity is critical
What is the storage buffer for RNase Inhibitor?
- 20 mM HEPES-KOH
- 0.1 mM EDTA
- 50 mM KCl
- 8 mM DTT
- 50% glycerol, pH 7.6
Which RNase types is RNase inhibitor effective against?
RNase types A, B and C.
When should RNase Inhibitor be added to a reaction?
Prior to the addition of any RNA that could be digested by RNases present in any of the components.
What is the optimal temperature for RNase Inhibitor?
RNase Inhibitor is active ≤ 55C.
Do I need to add the RNase Inhibitor when using purified RNA?
While it is not essential, we have demonstrated a significant increase in yield when RNase Inhibitor is added to RNA-based reactions.
What origin is Watchmaker’s RNase Inhibitor from?
The Watchmaker RNase Inhibitor is murine in origin.
Is DTT required for RNase inhibitor activity?
The absence of two cysteines present in human and porcine RNase inhibitors make this murine version much more suitable for low-DTT applications.
Can RNase Inhibitor be inactivated?
RNase Inhibitor can be heat-inactivated by incubating at 70°C for 20 minutes.
How much RNase inhibitor should be used?
We recommended 1 U/μL of RNase Inhibitor per assay.
What is the extinction coefficient for RNase Inhibitor?
The extinction coefficient for RNase Inhibitor is 0.8 (mL/(mg*cm). If measuring protein concentration (A280), we recommend you use the protein’s extinction coefficient to ensure an accurate reading.
What are the recommended reaction setup conditions?
Please reference our technical guide for instructions for use.
Do you offer custom formats?
Yes! Watchmaker offers customer fills, packaging, concentrations, and labeling – including private label – designed to meet your unique needs. We offer flexible terms to serve organizations of any size, and our right-sized processes enable rapid turnaround time on customization. Please contact sales@watchmakergenomics.com to learn more about our capabilities.
Are you ISO 13485-certified?
Yes! Our Quality Management System has achieved ISO 13485:2016 Certification. 我们的证书被授予设计、开发、制造、合同制造和支持在医学研究中的基因组学应用的高性能试剂。Download the certificate here.
